Dernière mise à jour le 12/05/2021

Domaine : Sciences du vivant   -   Thématique(s) : Microbiologie, génétique moléculaire

Stages courts

Epigenetic training course: « Initiation to chromatinandmethylated DNA immunoprecipitation»

Chromatin and DNA modifications, i.e. epigenetic modifications, are involved in the control of gene expression. This course provides a theoretical introduction to the field of epigenetics as well as a practical training to learn the main bases of the methods for studying these epigenetic modifications.

Dates : Lieu : Campus Pierre et Marie Curie – Paris (Jussieu) Tarif : 2185 €
Modalité : Présentiel

GOALS

  • Acquire the theoretical and practical bases necessary to analyse chromatin, chromatinian proteins and DNA methylation using chromatin immuno-precipitation (ChIP) and DNA methylation (MeDIP).

PUBLIC AND PRE-REQUISITE

Public :Technicians, engineers, researchers, teachers-researchers, doctoral students.

Pre-requisite: Basic knowledges in molecular biology, bachelor level.

PROGRAM

Lectures

  • Epigenetics and chromatin: post-translational modifications of histones, chromatin remodelling complexes.
  • Methylation and hydroxymethylation of DNA.
  • Epigenetic diseases.
  • Methods to analyse epigenetic modifications.
  • qPCR for ChIP and MeDIP.
  • Introduction to ChIP-seq.

Practical training

  • Chromatin and methylated DNA immunoprecipitation experiments from 3T3 cells (cross-linking, chromatin extraction and fragmentation, DNA extraction and fragmentation, immunoprecipitation, qPCR, analysis of results).

MEthodS

  • Lectures.
  • Practical training.
  • Teaching materials, bibliography and documentation are given to the participants.

EVALUATION METHODS

Statement of accomplishment.

HIGHLIGHTS

  • Teaching method adapted to acquire the strategic tools.
  • Teaching of the latest developments in epigenetics.
  • Instructors: Professor of Genetics and Researcher, experts in epigenetics.
  • Internship organized in collaboration with Diagenode

Pour candidater

Vous êtes une entreprise et souhaitez un accompagnement pour la construction de votre projet sur-mesure ou une proposition intra pour cette formation ?

Contactez-nous

Responsable(s)
pédagogique

Frédérique Peronnet

Voir le CV

Informations

Competency Development Action Category: (Article L6313-1 of the Labour Code)

Training action
Number of participants: from  8 to 16.
Language:The course can be provided in english or french upon request.

Contact

biosciences-fc@sorbonne-universite.fr

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